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ERITHACUS SOFTWARE LIMITED grafit 5.0
Grafit 5.0, supplied by ERITHACUS SOFTWARE LIMITED, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/grafit+5%2E0/grafit+5/pm40541648-101-17-19
Average 90 stars, based on 1 article reviews
grafit 5.0 - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Activity Assay:

Article Title: Fluorescent substrates enable specific detection and structure-function insights into human aldehyde dehydrogenase isoforms.
Article Snippet: Aldehyde dehydrogenase (ALDH) isoforms are widely used as biomarkers and potential drug targets in cancer research.. Quantitation of ALDH enzymatic activity in biological samples often relies on the use of commercially available assays that are quite unspecific and do not discriminate between the various ALDH isoforms.. The availability of highly purified recombinant ALDH isoforms allowed us to perform a full kinetic characterization of ALDH isoforms with fluorogenic substrates, BODIPYTM-aminoacetaldehyde (BAAA), the ALDEFLUORTM assay reagent, and two naphthaldedyde derivatives, 6-methoxy-2-naphthaldehyde (MONAL-62) and 7-methoxy-1naphthaldehyde (MONAL-71).

Article Title: Exploring the activity of the putative Δ6-desaturase and its role in bloodstream form life-cycle transitions in Trypanosoma brucei.
Article Snippet: EC50 values were determined using a 4-parameter non-linear logistic regression equation using GraFit 5.0 (Erithacus Software).

Article Title: ALDH1 subtype-specific inhibitor targets key cell populations in triple negative breast cancer
Article Snippet: The IC 50 values were calculated at saturating concentrations of substrate by fitting the initial rates to the appropriate equation using GraFit 5.0 (Erithacus Software) and the values were given as the mean ± standard error.

Article Title: Exploring the activity of the putative Δ6-desaturase and its role in bloodstream form life-cycle transitions in Trypanosoma brucei.
Article Snippet: Data analysis was carried using GraFit 5.0 (Erithacus Software). (DOCX) S20 Fig. SLS1 and SLS4 sequences identification via SWATH MS. SLS4 (A) and SLS1 (B) protein sequence are reported as presented in the proteomics analytical software MASCOT.

Article Title: Targeting Retinaldehyde Dehydrogenases to Enhance Temozolomide Therapy in Glioblastoma
Article Snippet: EC 50 values were calculated by nonlinear fitting of the obtained data to a sigmoidal plot using GraFit 5.0 (Erithacus software, http://www.erithacus.com/ , accessed on 26 August 2024), with the following 4-parameter Equation (1): (1) y = r a n g e 1 + x E C 50 s + b a c k g r o u n d where “ y ” is the percentage of viable cells, “ x ” is the concentration of the compound, “ background ” is the minimum y value, range is the fitted uninhibited value minus the background and “ s ” is a slope factor.

Article Title: Expression and Characterization of a Rice β-Xylosidase with Xylooligosaccharide Hydrolysis and Transglycosylation Activities
Article Snippet: Substrate saturation curves were fitted to Michaelis–Menten curves by nonlinear regression in GraFit 5.0 (Erithacus Software) for 4NPXyl and XOS, while Hanes–Woolf plots were used for fitting 4NPAra f and 4NPAra p data to obtain kinetic parameters since the substrate concentration could not be increased high enough to cover the K m values (data was not shown).

Article Title: Expression and Characterization of a Rice β-Xylosidase with Xylooligosaccharide Hydrolysis and Transglycosylation Activities.
Article Snippet: Substrate saturation curves were fitted to Michaelis−Menten curves by nonlinear regression in GraFit 5.0 (Erithacus Software) for 4NPXyl and XOS, while Hanes−Woolf plots were used for fitting 4NPAraf and 4NPArap data to obtain kinetic parameters since the substrate concentration could not be increased high enough to cover the Km values (data was not shown).

Concentration Assay:

Article Title: Fluorescent substrates enable specific detection and structure-function insights into human aldehyde dehydrogenase isoforms.
Article Snippet: Aldehyde dehydrogenase (ALDH) isoforms are widely used as biomarkers and potential drug targets in cancer research.. Quantitation of ALDH enzymatic activity in biological samples often relies on the use of commercially available assays that are quite unspecific and do not discriminate between the various ALDH isoforms.. The availability of highly purified recombinant ALDH isoforms allowed us to perform a full kinetic characterization of ALDH isoforms with fluorogenic substrates, BODIPYTM-aminoacetaldehyde (BAAA), the ALDEFLUORTM assay reagent, and two naphthaldedyde derivatives, 6-methoxy-2-naphthaldehyde (MONAL-62) and 7-methoxy-1naphthaldehyde (MONAL-71).

Article Title: Exploring the activity of the putative Δ6-desaturase and its role in bloodstream form life-cycle transitions in Trypanosoma brucei.
Article Snippet: EC50 values were determined using a 4-parameter non-linear logistic regression equation using GraFit 5.0 (Erithacus Software).

Article Title: ALDH1 subtype-specific inhibitor targets key cell populations in triple negative breast cancer
Article Snippet: The IC 50 values were calculated at saturating concentrations of substrate by fitting the initial rates to the appropriate equation using GraFit 5.0 (Erithacus Software) and the values were given as the mean ± standard error.

Article Title: Exploring the activity of the putative Δ6-desaturase and its role in bloodstream form life-cycle transitions in Trypanosoma brucei.
Article Snippet: Data analysis was carried using GraFit 5.0 (Erithacus Software). (DOCX) S20 Fig. SLS1 and SLS4 sequences identification via SWATH MS. SLS4 (A) and SLS1 (B) protein sequence are reported as presented in the proteomics analytical software MASCOT.

Article Title: Targeting Retinaldehyde Dehydrogenases to Enhance Temozolomide Therapy in Glioblastoma
Article Snippet: EC 50 values were calculated by nonlinear fitting of the obtained data to a sigmoidal plot using GraFit 5.0 (Erithacus software, http://www.erithacus.com/ , accessed on 26 August 2024), with the following 4-parameter Equation (1): (1) y = r a n g e 1 + x E C 50 s + b a c k g r o u n d where “ y ” is the percentage of viable cells, “ x ” is the concentration of the compound, “ background ” is the minimum y value, range is the fitted uninhibited value minus the background and “ s ” is a slope factor.

Article Title: Expression and Characterization of a Rice β-Xylosidase with Xylooligosaccharide Hydrolysis and Transglycosylation Activities
Article Snippet: Substrate saturation curves were fitted to Michaelis–Menten curves by nonlinear regression in GraFit 5.0 (Erithacus Software) for 4NPXyl and XOS, while Hanes–Woolf plots were used for fitting 4NPAra f and 4NPAra p data to obtain kinetic parameters since the substrate concentration could not be increased high enough to cover the K m values (data was not shown).

Article Title: Expression and Characterization of a Rice β-Xylosidase with Xylooligosaccharide Hydrolysis and Transglycosylation Activities.
Article Snippet: Substrate saturation curves were fitted to Michaelis−Menten curves by nonlinear regression in GraFit 5.0 (Erithacus Software) for 4NPXyl and XOS, while Hanes−Woolf plots were used for fitting 4NPAraf and 4NPArap data to obtain kinetic parameters since the substrate concentration could not be increased high enough to cover the Km values (data was not shown).

Data-independent acquisition:

Article Title: Fluorescent substrates enable specific detection and structure-function insights into human aldehyde dehydrogenase isoforms.
Article Snippet: Aldehyde dehydrogenase (ALDH) isoforms are widely used as biomarkers and potential drug targets in cancer research.. Quantitation of ALDH enzymatic activity in biological samples often relies on the use of commercially available assays that are quite unspecific and do not discriminate between the various ALDH isoforms.. The availability of highly purified recombinant ALDH isoforms allowed us to perform a full kinetic characterization of ALDH isoforms with fluorogenic substrates, BODIPYTM-aminoacetaldehyde (BAAA), the ALDEFLUORTM assay reagent, and two naphthaldedyde derivatives, 6-methoxy-2-naphthaldehyde (MONAL-62) and 7-methoxy-1naphthaldehyde (MONAL-71).

Article Title: Exploring the activity of the putative Δ6-desaturase and its role in bloodstream form life-cycle transitions in Trypanosoma brucei.
Article Snippet: EC50 values were determined using a 4-parameter non-linear logistic regression equation using GraFit 5.0 (Erithacus Software).

Article Title: ALDH1 subtype-specific inhibitor targets key cell populations in triple negative breast cancer
Article Snippet: The IC 50 values were calculated at saturating concentrations of substrate by fitting the initial rates to the appropriate equation using GraFit 5.0 (Erithacus Software) and the values were given as the mean ± standard error.

Article Title: Exploring the activity of the putative Δ6-desaturase and its role in bloodstream form life-cycle transitions in Trypanosoma brucei.
Article Snippet: Data analysis was carried using GraFit 5.0 (Erithacus Software). (DOCX) S20 Fig. SLS1 and SLS4 sequences identification via SWATH MS. SLS4 (A) and SLS1 (B) protein sequence are reported as presented in the proteomics analytical software MASCOT.

Article Title: Targeting Retinaldehyde Dehydrogenases to Enhance Temozolomide Therapy in Glioblastoma
Article Snippet: EC 50 values were calculated by nonlinear fitting of the obtained data to a sigmoidal plot using GraFit 5.0 (Erithacus software, http://www.erithacus.com/ , accessed on 26 August 2024), with the following 4-parameter Equation (1): (1) y = r a n g e 1 + x E C 50 s + b a c k g r o u n d where “ y ” is the percentage of viable cells, “ x ” is the concentration of the compound, “ background ” is the minimum y value, range is the fitted uninhibited value minus the background and “ s ” is a slope factor.

Article Title: Expression and Characterization of a Rice β-Xylosidase with Xylooligosaccharide Hydrolysis and Transglycosylation Activities
Article Snippet: Substrate saturation curves were fitted to Michaelis–Menten curves by nonlinear regression in GraFit 5.0 (Erithacus Software) for 4NPXyl and XOS, while Hanes–Woolf plots were used for fitting 4NPAra f and 4NPAra p data to obtain kinetic parameters since the substrate concentration could not be increased high enough to cover the K m values (data was not shown).

Article Title: Expression and Characterization of a Rice β-Xylosidase with Xylooligosaccharide Hydrolysis and Transglycosylation Activities.
Article Snippet: Substrate saturation curves were fitted to Michaelis−Menten curves by nonlinear regression in GraFit 5.0 (Erithacus Software) for 4NPXyl and XOS, while Hanes−Woolf plots were used for fitting 4NPAraf and 4NPArap data to obtain kinetic parameters since the substrate concentration could not be increased high enough to cover the Km values (data was not shown).

Sequencing:

Article Title: Fluorescent substrates enable specific detection and structure-function insights into human aldehyde dehydrogenase isoforms.
Article Snippet: Aldehyde dehydrogenase (ALDH) isoforms are widely used as biomarkers and potential drug targets in cancer research.. Quantitation of ALDH enzymatic activity in biological samples often relies on the use of commercially available assays that are quite unspecific and do not discriminate between the various ALDH isoforms.. The availability of highly purified recombinant ALDH isoforms allowed us to perform a full kinetic characterization of ALDH isoforms with fluorogenic substrates, BODIPYTM-aminoacetaldehyde (BAAA), the ALDEFLUORTM assay reagent, and two naphthaldedyde derivatives, 6-methoxy-2-naphthaldehyde (MONAL-62) and 7-methoxy-1naphthaldehyde (MONAL-71).

Article Title: Exploring the activity of the putative Δ6-desaturase and its role in bloodstream form life-cycle transitions in Trypanosoma brucei.
Article Snippet: EC50 values were determined using a 4-parameter non-linear logistic regression equation using GraFit 5.0 (Erithacus Software).

Article Title: ALDH1 subtype-specific inhibitor targets key cell populations in triple negative breast cancer
Article Snippet: The IC 50 values were calculated at saturating concentrations of substrate by fitting the initial rates to the appropriate equation using GraFit 5.0 (Erithacus Software) and the values were given as the mean ± standard error.

Article Title: Exploring the activity of the putative Δ6-desaturase and its role in bloodstream form life-cycle transitions in Trypanosoma brucei.
Article Snippet: Data analysis was carried using GraFit 5.0 (Erithacus Software). (DOCX) S20 Fig. SLS1 and SLS4 sequences identification via SWATH MS. SLS4 (A) and SLS1 (B) protein sequence are reported as presented in the proteomics analytical software MASCOT.

Article Title: Targeting Retinaldehyde Dehydrogenases to Enhance Temozolomide Therapy in Glioblastoma
Article Snippet: EC 50 values were calculated by nonlinear fitting of the obtained data to a sigmoidal plot using GraFit 5.0 (Erithacus software, http://www.erithacus.com/ , accessed on 26 August 2024), with the following 4-parameter Equation (1): (1) y = r a n g e 1 + x E C 50 s + b a c k g r o u n d where “ y ” is the percentage of viable cells, “ x ” is the concentration of the compound, “ background ” is the minimum y value, range is the fitted uninhibited value minus the background and “ s ” is a slope factor.

Article Title: Expression and Characterization of a Rice β-Xylosidase with Xylooligosaccharide Hydrolysis and Transglycosylation Activities
Article Snippet: Substrate saturation curves were fitted to Michaelis–Menten curves by nonlinear regression in GraFit 5.0 (Erithacus Software) for 4NPXyl and XOS, while Hanes–Woolf plots were used for fitting 4NPAra f and 4NPAra p data to obtain kinetic parameters since the substrate concentration could not be increased high enough to cover the K m values (data was not shown).

Article Title: Expression and Characterization of a Rice β-Xylosidase with Xylooligosaccharide Hydrolysis and Transglycosylation Activities.
Article Snippet: Substrate saturation curves were fitted to Michaelis−Menten curves by nonlinear regression in GraFit 5.0 (Erithacus Software) for 4NPXyl and XOS, while Hanes−Woolf plots were used for fitting 4NPAraf and 4NPArap data to obtain kinetic parameters since the substrate concentration could not be increased high enough to cover the Km values (data was not shown).

Software:

Article Title: Fluorescent substrates enable specific detection and structure-function insights into human aldehyde dehydrogenase isoforms.
Article Snippet: Aldehyde dehydrogenase (ALDH) isoforms are widely used as biomarkers and potential drug targets in cancer research.. Quantitation of ALDH enzymatic activity in biological samples often relies on the use of commercially available assays that are quite unspecific and do not discriminate between the various ALDH isoforms.. The availability of highly purified recombinant ALDH isoforms allowed us to perform a full kinetic characterization of ALDH isoforms with fluorogenic substrates, BODIPYTM-aminoacetaldehyde (BAAA), the ALDEFLUORTM assay reagent, and two naphthaldedyde derivatives, 6-methoxy-2-naphthaldehyde (MONAL-62) and 7-methoxy-1naphthaldehyde (MONAL-71).

Article Title: Exploring the activity of the putative Δ6-desaturase and its role in bloodstream form life-cycle transitions in Trypanosoma brucei.
Article Snippet: EC50 values were determined using a 4-parameter non-linear logistic regression equation using GraFit 5.0 (Erithacus Software).

Article Title: ALDH1 subtype-specific inhibitor targets key cell populations in triple negative breast cancer
Article Snippet: The IC 50 values were calculated at saturating concentrations of substrate by fitting the initial rates to the appropriate equation using GraFit 5.0 (Erithacus Software) and the values were given as the mean ± standard error.

Article Title: Exploring the activity of the putative Δ6-desaturase and its role in bloodstream form life-cycle transitions in Trypanosoma brucei.
Article Snippet: Data analysis was carried using GraFit 5.0 (Erithacus Software). (DOCX) S20 Fig. SLS1 and SLS4 sequences identification via SWATH MS. SLS4 (A) and SLS1 (B) protein sequence are reported as presented in the proteomics analytical software MASCOT.

Article Title: Targeting Retinaldehyde Dehydrogenases to Enhance Temozolomide Therapy in Glioblastoma
Article Snippet: EC 50 values were calculated by nonlinear fitting of the obtained data to a sigmoidal plot using GraFit 5.0 (Erithacus software, http://www.erithacus.com/ , accessed on 26 August 2024), with the following 4-parameter Equation (1): (1) y = r a n g e 1 + x E C 50 s + b a c k g r o u n d where “ y ” is the percentage of viable cells, “ x ” is the concentration of the compound, “ background ” is the minimum y value, range is the fitted uninhibited value minus the background and “ s ” is a slope factor.

Article Title: Expression and Characterization of a Rice β-Xylosidase with Xylooligosaccharide Hydrolysis and Transglycosylation Activities
Article Snippet: Substrate saturation curves were fitted to Michaelis–Menten curves by nonlinear regression in GraFit 5.0 (Erithacus Software) for 4NPXyl and XOS, while Hanes–Woolf plots were used for fitting 4NPAra f and 4NPAra p data to obtain kinetic parameters since the substrate concentration could not be increased high enough to cover the K m values (data was not shown).

Article Title: Expression and Characterization of a Rice β-Xylosidase with Xylooligosaccharide Hydrolysis and Transglycosylation Activities.
Article Snippet: Substrate saturation curves were fitted to Michaelis−Menten curves by nonlinear regression in GraFit 5.0 (Erithacus Software) for 4NPXyl and XOS, while Hanes−Woolf plots were used for fitting 4NPAraf and 4NPArap data to obtain kinetic parameters since the substrate concentration could not be increased high enough to cover the Km values (data was not shown).



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